The Science — RESCUESKIN LABORATORIES
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The science behind our adult human stemcell skincare technology

The science behind our adult human stemcell skincare technology

01.

Extracting Adult Human Mesenchymal Stem Cells (MSCS)

Adult MSC’s are obtained by human bone marrow aspiration from healthy screened donors, typically between 20-25 years old. The process is painless, and whatever the donor gives is regenerated within a matter of days.

By mimicking your own cell’s regenerative process on a cellular level, you support the skin you can’t see, and the skin you can see improves dramatically.

02.

Growing MSCS To Produce Stem Cell Derivatives 

Starting with 500,000 MSC’s (about 16 oz - can of coke), we culture these MSC’s through 10 passages in a 3 month process that is monitored daily by a team of scientists.

Those initial 500,000 cells multiple to 10 trillion which produces 195 liters of stem cells. We then remove those cells using various tiny filters. The MSC is not extracted or broken open. Instead, exosomes, peptides, proteins, cytokines, and enzymes that were released during their growth are collected using even smaller filters and become the basis for our stem cells derived actives.

03. 

Quality Control & Skin Cell Testing

Even though the cells have already passed numerous tests for safety, we repeatedly conduct microbiology at every growth stage, as well as nutrient and metabolic testing; to ensure electrolytes, glucose, glutathione, glutamate, lactate, ammonia, and LDH are at acceptable levels. Cell morphology (how the cells look and function) is also examined at every tissue culture media change and passage.

Finally, we perform something known as a "functional challenge" against American Type Tissue Culture sourced human dermal fibroblasts to ensure our cells and their derivatives are of the highest quality. The functional challenge test is also referred to as a "gap assay," in which our stem cell-derived media is used to culture (grow) two separate human dermal fibroblast (skin) cell lines in Petri dishes.

Once the cells and our media have been combined in the Petri dish, we draw a gap using a swab. The gap replicates what would happen if the skin was cut or damaged. We then measure how quickly the gap is filled, which includes growth rates of the human dermal fibroblasts, as well as pro-collagen production. This is one of the things that makes our product so unique.

A Biological Master File has been accepted by the Food & Drug Administration (FDA) that includes the proprietary components of the production media, the source of cells, the tissue culture procedures, and the multiple tests run throughout this process, as well as the test results.

04. 

Your Skin and MSCS

So how does this interact with your skin? Simply put, stem cells are rapidly dividing cells in your body that have the ability to product more stem cells with specialized functions. Human stem cells contain proteins and amino acids that signal your body’s cells to rejuvenate. As a building block for cell rejuventation with natural antioxidant properties, they promote cell turnover and increase collagen production. As a result, you experience improved skin texture and tone along with fewer lines and wrinkles. See it for yourself through our testimonials.

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01. 

Extracting Adult Human Mesenchymal Stem Cells (MSCS)

Adult MSC’s are obtained by human bone marrow aspiration from healthy screened donors, typically between 20-25 years old. The process is painless, and whatever the donor gives is regenerated within a matter of days.

By mimicking your own cell’s regenerative process on a cellular level, you support the skin you can’t see, and the skin you can see improves dramatically.

02. 

Growing MSCS To Produce Stem Cell Derivatives 

Starting with 500,000 MSC’s (about 16 oz - can of coke), we culture these MSC’s through 10 passages in a 3 month process that is monitored daily by a team of scientists. Those initial 500,000 cells multiple to 10 trillion which produces 195 liters of stem cells.

We then remove those cells using various tiny filters. The MSC is not extracted or broken open. Instead, exosomes, peptides, proteins, cytokines, and enzymes that were released during their growth are collected using even smaller filters and become the basis for our stem cells derived actives.

03. 

Quality Control & Skin Cell Testing

Even though the cells have already passed numerous tests for safety, we repeatedly conduct microbiology at every growth stage, as well as nutrient and metabolic testing; to ensure electrolytes, glucose, glutathione, glutamate, lactate, ammonia, and LDH are at acceptable levels. Cell morphology (how the cells look and function) is also examined at every tissue culture media change and passage.

Finally, we perform something known as a "functional challenge" against American Type Tissue Culture sourced human dermal fibroblasts to ensure our cells and their derivatives are of the highest quality. The functional challenge test is also referred to as a "gap assay," in which our stem cell-derived media is used to culture (grow) two separate human dermal fibroblast (skin) cell lines in Petri dishes.

Once the cells and our media have been combined in the Petri dish, we draw a gap using a swab. The gap replicates what would happen if the skin was cut or damaged. We then measure how quickly the gap is filled, which includes growth rates of the human dermal fibroblasts, as well as pro-collagen production. This is one of the things that makes our product so unique.

A Biological Master File has been accepted by the Food & Drug Administration (FDA) that includes the proprietary components of the production media, the source of cells, the tissue culture procedures, and the multiple tests run throughout this process, as well as the test results.

04. 

Your Skin and MSCS

So how does this interact with your skin? Simply put, stem cells are rapidly dividing cells in your body that have the ability to product more stem cells with specialized functions. Human stem cells contain proteins and amino acids that signal your body’s cells to rejuvenate. As a building block for cell rejuventation with natural antioxidant properties, they promote cell turnover and increase collagen production. As a result, you experience improved skin texture and tone along with fewer lines and wrinkles. See it for yourself through our testimonials.